Publications
2009
Coulombe PA, Kerns ML, Fuchs E. Epidermolysis bullosa simplex: a paradigm for disorders of tissue fragility. J Clin Invest. 2009 July;119(7):1784-1793.
2008
Huang SM, Lee H, Chung MK, Park U, Yu YY, Bradshaw HB, Coulombe PA, Walker JM, Caterina MJ. Overexpressed transient receptor potential vanilloid 3 ion channels in skin keratinocytes modulate pain sensitivity via prostaglandin E2. J Neurosci. 2008 Dec 17;28(51):13727-37.
Gu LH, Coulombe PA. Hedgehog signaling, keratin 6 induction, and sebaceous gland morphogenesis: implications for pachyonychia congenita and related conditions. Am J Pathol. 2008 Sep;173(3):752-61. Epub 2008 Aug 7.
Larouche D, Tong X, Fradette J, Coulombe PA, Germain L. Vibrissa hair bulge houses two populations of skin epithelial stem cells distinct by their keratin profile.
FASEB J. 2008 May;22(5):1404-15. Epub 2007 Dec 27.
2007
Kim S, Kellner J, Lee CH, Coulombe PA.
Interaction between the keratin cytoskeleton and eEF1Bgamma affects protein synthesis in epithelial cells.
Nat Struct Mol Biol. 2007 Oct;14(10):982-3. Epub 2007 Sep 30.
Kerns ML, DePianto D, Dinkova-Kostova AT, Talalay P, Coulombe PA. Reprogramming of keratin biosynthesis by sulforaphane restores skin integrity in epidermolysis bullosa simplex. Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14460-5. Epub 2007 Aug 27.
Kim S, Coulombe PA.
Intermediate filament scaffolds fulfill mechanical, organizational, and signaling functions in the cytoplasm.
Genes Dev. 2007 Jul 1;21(13):1581-97. Review.
Leung CT, Coulombe PA, Reed RR.
Contribution of olfactory neural stem cells to tissue maintenance and regeneration.
Nat Neurosci. 2007 Jun;10(6):720-6. Epub 2007 Apr 29.
Gu LH, Coulombe PA.
Keratin function in skin epithelia: a broadening palette with surprising shades.
Curr Opin Cell Biol. 2007 Feb;19(1):13-23. Epub 2006 Dec 18. Review.
Gu LH, Coulombe PA.
Keratin expression provides novel insight into the morphogenesis and function of the companion layer in hair follicles.
J Invest Dermatol. 2007 May;127(5):1061-73. Epub 2006 Dec 14.
2006
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW. New consensus nomenclature for mammalian keratins.
J Cell Biol. 2006 Jul 17;174(2):169-74. Epub 2006 Jul 10.
Kim S, Wong P, Coulombe PA.
A keratin cytoskeletal protein regulates protein synthesis and epithelial cell growth.
Nature. 2006 May 18;441(7091):362-5.
Tong X, Coulombe PA.
Keratin 17 modulates hair follicle cycling in a TNFalpha-dependent fashion. Genes Dev. 2006 May 15;20(10):1353-64.
2005
Beaudoin GM 3rd, Sisk JM, Coulombe PA, Thompson CC.
Hairless triggers reactivation of hair growth by promoting Wnt signaling.
Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14653-8. Epub 2005 Sep 29.
Bianchi N, Depianto D, McGowan K, Gu C, Coulombe PA. Exploiting the keratin 17 gene promoter to visualize live cells in epithelial appendages of mice.
Mol Cell Biol. 2005 Aug;25(16):7249-59.
Bernot KM, Lee CH, Coulombe PA.
A small surface hydrophobic stripe in the coiled-coil domain of type I keratins mediates tetramer stability.
J Cell Biol. 2005 Mar 14;168(6):965-74.
Gu LH, Coulombe PA.
Defining the properties of the nonhelical tail domain in type II keratin 5: insight from a bullous disease-causing mutation.
Mol Biol Cell. 2005 Mar;16(3):1427-38. Epub 2005 Jan 12.
Wong P, Domergue R, Coulombe PA.
Overcoming functional redundancy to elicit pachyonychia congenita-like nail lesions in transgenic mice.
Mol Cell Biol. 2005 Jan;25(1):197-205.
2004
Coulombe PA, Tong X, Mazzalupo S, Wang Z, Wong P.
Great promises yet to be fulfilled: defining keratin intermediate filament function in vivo.
Eur J Cell Biol. 2004 Dec;83(11-12):735-46. Review.
Bernot KM, Coulombe PA, Wong P.
Skin: an ideal model system to study keratin genes and proteins.
Methods Cell Biol. 2004;78:453-87.
Callahan CA, Ofstad T, Horng L, Wang JK, Zhen HH, Coulombe PA, Oro AE. MIM/BEG4, a Sonic hedgehog-responsive gene that potentiates Gli-dependent transcription.
Genes Dev. 2004 Nov 15;18(22):2724-9.
Omary MB, Coulombe PA, McLean WH. Intermediate filament proteins and their associated diseases.
N Engl J Med. 2004 Nov 11;351(20):2087-100. Review.
DePianto D, Coulombe PA (2004). Intermediate filaments and tissue repair. Exp Cell Res. 2004 Nov 15;301(1):68-76. Review.
Presland RB, Coulombe PA, Eckert RL, Mao-Qiang M, Feingold KR, Elias PM.
Barrier function in transgenic mice overexpressing K16, involucrin, and filaggrin in the suprabasal epidermis.
J Invest Dermatol. 2004 Sep;123(3):603-6.
{{PubMed|15303099 }Coulombe PA, Wong P. Cytoplasmic intermediate filaments revealed as dynamic and multipurpose scaffolds. Nat Cell Biol. 2004 Aug;6(8):699-706. Review.
Zarach JM, Beaudoin GM 3rd, Coulombe PA, Thompson CC.
The co-repressor hairless has a role in epithelial cell differentiation in the skin.
Development. 2004 Sep;131(17):4189-200. Epub 2004 Jul 27.
Tong X, Coulombe PA.
A novel mouse type I intermediate filament gene, keratin 17n (K17n), exhibits preferred expression in nail tissue.
J Invest Dermatol. 2004 Apr;122(4):965-70.
2003
Wang Z, Wong P, Langbein L, Schweizer J, Coulombe PA.
Type II epithelial keratin 6hf (K6hf) is expressed in the companion layer, matrix, and medulla in anagen-stage hair follicles.
J Invest Dermatol. 2003 Dec;121(6):1276-82.
Wong P, Coulombe PA.
Loss of keratin 6 (K6) proteins reveals a function for intermediate filaments during wound repair.
J Cell Biol. 2003 Oct 27;163(2):327-37. Epub 2003 Oct 20.
Yamada S, Wirtz D, Coulombe PA.
The mechanical properties of simple epithelial keratins 8 and 18: discriminating between interfacial and bulk elasticities.
J Struct Biol. 2003 Jul;143(1):45-55.
Coulombe PA.
Wound epithelialization: accelerating the pace of discovery.
J Invest Dermatol. 2003 Aug;121(2):219-30. Review. No abstract available.
Tong X, Coulombe PA.
Mouse models of alopecia: identifying structural genes that are baldly needed.
Trends Mol Med. 2003 Feb;9(2):79-84. Review.
Mazzalupo S, Wong P, Martin P, Coulombe PA.
Role for keratins 6 and 17 during wound closure in embryonic mouse skin. Dev Dyn. 2003 Feb;226(2):356-65.
2002
Bernot KM, Coulombe PA, McGowan KM.
Keratin 16 expression defines a subset of epithelial cells during skin morphogenesis and the hair cycle.
J Invest Dermatol. 2002 Nov;119(5):1137-49.
Coulombe PA.
A new fold on an old story: attachment of intermediate filaments to desmosomes.
Nat Struct Biol. 2002 Aug;9(8):560-2. No abstract available.
McGowan KM, Tong X, Colucci-Guyon E, Langa F, Babinet C, Coulombe PA.
Keratin 17 null mice exhibit age- and strain-dependent alopecia.
Genes Dev. 2002 Jun 1;16(11):1412-22.
Mazzalupo S, Wawersik MJ, Coulombe PA.
An ex vivo assay to assess the potential of skin keratinocytes for wound epithelialization.
J Invest Dermatol. 2002 May;118(5):866-70.
Yamada S, Wirtz D, Coulombe PA.
Pairwise assembly determines the intrinsic potential for self-organization and mechanical properties of keratin filaments.
Mol Biol Cell. 2002 Jan;13(1):382-91.
Coulombe PA, Omary MB.
'Hard' and 'soft' principles defining the structure, function and regulation of keratin intermediate filaments.
Curr Opin Cell Biol. 2002 Feb;14(1):110-22. Review.
2001
Coulombe, P. A. , Ma L, Yamada S., Wawersik M (2001). Intermediate filaments at a glance. Journal of Cell Science.
Bousquet, O., Ma, L., Yamada, S., Gu, C., Idei, T., Takahashi, K., Wirtz, D., Coulombe, P. A. (2001). The non-helical tail domain of keratin14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro. Journal of Cell Biology 155: 747-53.
Wawersik M, Mazzalupo, S, Nguyen D, Coulombe, P. A. (2001). Increased Levels of Keratin 16 Alters the Epithelialization Potential of Skin Keratinocytes In Vivo and Ex Vivo. Molecular Biology of the Cell 12: 3439-50
Ma L, Yamada S, Wirtz D, Coulombe, P. A. (2001). A "hot spot" mutation alters the mechanical properties of keratin filament networks. Nature Cell Biology 3: 503-6.
Mazzalupo S, Coulombe, P. A. (2001). A Reporter Transgene Based on a Human Keratin 6 Gene Promoter Is Specifically Expressed in the Periderm of Mouse Embryos. Mechanisms of Development 100: 65-69.
2000
Wawersik MJ, Coulombe, P. A. (2000). Forced expression of keratin 16 alters adhesion and differentiation properties of mouse skin keratinocytes. Molecular Biology of the Cell 11: 3315-27.
Coulombe, P. A., Bousquet O, Ma L, Yamada S, Wirtz D (2000). The "Ins" and "Outs" of intermediate filament organization. Trends in Cell Biology 10: 420-8.
Coulombe, P. A., McGowan K (2000). Morphogenesis of Mammalian Skin. In: Cell Polarity: Frontiers in Molecular Biology. Edited by D.G. Drubin. Oxford University Press, Oxford, England.
Wong P, Colucci-Guyon E, Gu C, Takahashi K, Babinet C, Coulombe, P. A. (2000). Introducing a null mutation in the mouse K6a and K6b genes reveals their essential structural role in oral mucosa epithelia. Journal of Cell Biology 150: 921-8.
McGowan K, Coulombe, P. A. (2000). The type I keratin 17 is expressed in the context of hard epithelia: Implications for the molecular pathogenesis of pachyonychia congenita. Journal of Investigative Dermatology 114: 1101-8. **cover article.
Steinbock FA, Nikolic B, Coulombe, P. A., Fuchs E, Traub P, Wiche G (2000). Dose-dependent linkage, assembly inhibition and disassembly of vimentin and cytokeratin 5/14 filaments through plectin's intermediate filament binding domain. Journal of Cell Science 113: 483-91.
1999
Takahashi K, Coulombe, P. A., Miyachi Y (1999). Using transgenic mouse models to study the pathogenesis of keratin-based inherited skin diseases. Journal of Dermatological Sciences 21: 73-95.
Paladini RD, Coulombe, P. A. (1999). The functional diversity of epidermal keratins revealed by the partial rescue of the keratin 14 null phenotype by keratin 16. Journal of Cell Biology 146: 1185-1201 **cover article.
Ma L, Xu J, Coulombe, P. A., Wirtz D (1999). Keratin filament suspensions show unique micromechanical properties. Journal of Biological Chemistry 274: 19145-51.
1998
Fradette J, Germain L, Sessaiah P, Coulombe, P. A. (1998). The type I keratin 19 shows distinct and context-dependent properties. Journal of Biological Chemistry, 273, 36176-84.
Hutton E, Paladini, RD, Yu QC, Yen M, Coulombe, P. A., Fuchs E (1998). Functional differences between keratins of stratified and simple epithelia. Journal of Cell Biology, 143, 487-99.
McGowan K, Coulombe, P. A. (1998). Onset of keratin 17 expression coincides with the definition of major epithelial lineages during skin development. Journal of Cell Biology, 143, 469-86.
Takahashi K, Yan B, Yamanishi K, Imamura S, Coulombe, P. A. (1998). The two functional keratin 6 genes of mouse are differentially regulated and evolved independently from their human orthologs. Genomics, 53, 170-183.
Munoz-Marmol AM, Strasser G, Isamat M, Coulombe, P. A., Yang Y, Roca X, Vela E, Mate JL, Coll J, Fernandez-Figueras MT, Navas-Palacio JJ, Ariza A, Fuchs E (1998). A dysfynctional desmin mutation in a patient with severe generalized myopathy. Proceedings of the National Academy of Science (USA) 95, 11312-17.
Paladini RD, Coulombe, P. A. (1998). Directed expression of keratin 16 to the progenitor basal cells of transgenic mouse skin delays skin maturation. Journal of Cell Biology, 142, 1035-51.
Miller SJ, Burke EM, Rader MD, Coulombe, P. A., Lavker RM (1998). Reconstitution of wounded porcine epidermis from follicular and apocrine stem cells. Journal of Investigative Dermatology, 110:13-19.
1997
McGowan K, Coulombe, P. A. (1997). The wound repair-associated keratins 6, 16 and 17: Insights into the role of intermediate filaments in specifying keratinocyte cytoarchitecture. In: Subcellular Biochemistry vol. 31: Intermediate Filaments (Herrmann H, Harris R, Eds.). Plenum Press, New York, pp. 173-204.
Coulombe, P. A. (1997). Towards a molecular definition of keratinocyte activation following acute injury to stratified epithelia. Biochemical and Biophysical Research Communications 236: 231-8.
Coulombe, P. A. (1997). Transgenic mice as a model for the study of inherited skin bullous diseases. Ann. Dermatol. Venerol. 124: 197-204.
Wawersik M, Paladini RD, Noensie E, Coulombe, P. A. (1997). A proline residue in the a-helical rod domain of type I keratin 16 destabilizes keratin heterotetramers. Journal of Biological Chemistry 272: 32557-65.
Takahashi K, Coulombe, P. A. (1997). Defining a region of the human K6a gene that confers inducible expression in stratified epithelial of transgenic mice. Journal of Biological Chemistry 272: 10979-85.
1996
Takahashi K, Coulombe, P. A. (1996). A mouse model with an inducible skin blistering phenotype. Proceedings of the National Academy of Science (USA) 93: 14776-81.
Bousquet O, Coulombe, P. A. (1996). The cytoskeleton: Missing links found? Current Biology 6: 1563-66.
Coulombe, P. A., Takahashi K (1996): The cellular mechanisms of skin keratinocyte migration into a wound site: An open question with important implications. Cell Vision - Journal of Analytical Morphology 3: 217-223.
Uttam J, Hutton E, Coulombe, P. A., Anton-Lampbrecht I, Yu QC, Gedde-Dahl T, Fine JD, Fuchs E (1996): The genetic basis for epidermolysis bullosa simplex with mottled pigmentation. Proceedings of the National Academy of Science (USA) 93: 9079-84.
Paladini R, Bravo NS, Takahashi K, Coulombe, P. A. (1996). Onset ot re-epithelialization after skin injury correlates with a reorganization of keratin filaments in wound edge keratinocytes: Defining a potential role for keratin 16. Journal of Cell Biology 132: 381-98.
1995
Coulombe, P. A., Bravo NS, Paladini RD, Nguyen D, Takahashi K (1995): Over-expression of human keratin 16 produces a distinct skin phenotype in transgenic mice. Biochemistry and Cell Biology 73 (9-10): 611-18.
Paladini R, Takahashi K, Gant TM, Coulombe, P. A. (1995). cDNA cloning and bacterial expression of the human type I keratin 16. Biochemical and Biophysical Research Communications 215: 517-23.
Takahashi K, Paladini R, Coulombe, P. A. (1995). Cloning and characterization of multiple human genes and cDNAs encoding highly-related type II keratin 6 isoforms. Journal of Biological Chemistry 270(31): 18581-18592.
Lowthert LA, Ku NO, Liao J, Coulombe, P. A., Omary MB (1995): Empigen BB: A useful detergent for solubilization and biochemical analysis of keratins. Biochemical and Biophysical Research Communications 206: 370-379.
1994
Coulombe, P. A., Fuchs (1994): Molecular mechanisms of keratin gene disorders and other inherited bullous diseases of the skin. In: "Molecular Mechanisms in Epithelial Cell Junctions: from Development to Disease". S. Citi, Editor. R.G. Landes Company, Austin (TX). pp. 259-284.
Takahashi K, Folmer J, Coulombe, P. A. (1994): Increased expression of keratin 16 causes anomalies in cytoarchitecture and keratinization in transgenic mouse skin. Journal of Cell Biology 127: 505-520.
1993
Coulombe, P. A., Fuchs E (1993): Epidermolysis Bullosa Simplex. Seminars in Dermatology 12: 173-190.
Coulombe, P. A. (1993): Cellular and molecular biology of keratins: Beginning a new era. Current Opinion in Cell Biology 5: 17-29.
